Title | A process for producing lipase |
---|---|
Project Leader | |
Name | Raja Noor Zaliha Raja Abd. Rahman |
Telephone No. | 0389466713 |
rnzaliha@biotech.upm.edu.my | |
Faculty | Biotechnology and Biomolecul Sciences |
IP Information | |
IP Type | Patent |
Filed Date | 20-05-2013 |
Application No. | PI2013700818 |
Country Filing | Malaysia |
Applicant | Universiti Putra Malaysia |
Granted Date | |
IP Number | - |
Abstract | |
A process for producing lipase by microorganisms on the industrial scale, wherein Escherichia coli microorganism harbouring lipase, preferably thermostable lipase, is fermented under batch or fed batch conditions, in a Luria Bertani (LB) broth supplemented with citric acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, NH4Cl and glucose, using a trace metal solution containing hydrochloric acid, FeSO4.7H2O, ZnSO4.7H20, CaCl2.H20, MnSO4.5H20, CuSO4.5H2O, AlCl.6H2O, and H3BO3, and a feeding nutrient solution containing glucose, yeast extract, MgSO4.H2O, KH2PO4, Citric Acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, and NH4Cl, at dissolved oxygen tension of 30-50 %, at temperature of 30-40 °C, while maintaining the pH between 6 and 8, with a glucose content of 15-25 g/L, with isopropylthiogalactoside. Figure 1-6 | |
Technology | Producing lipase |
Inventor | Raja Noor Zaliha Raja Abd. Rahman |
Statement of Novelty | |
Work Start Date | 01-01-0001 |
Work End Date | 01-01-0001 |
Classification | |
Commercialised Status | |
Faculty | Biotechnology and Biomolecul Sciences |