Title | Detection of Newcastle Disease Virus |
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Project Leader | |
Name | Khatijah Yusoff |
Telephone No. | 0389466024 |
kyusoff@biotech.upm.edu.my | |
Faculty | Biotechnology and Biomolecul Sciences |
IP Information | |
IP Type | Patent |
Filed Date | 27-11-2000 |
Application No. | PI20005526 |
Country Filing | Malaysia |
Applicant | Universiti Putra Malaysia |
Granted Date | |
IP Number | MY134891A |
Abstract | |
A method for detection of Newcastle disease virus in a biological sample comprising (a) the extraction of mRNA from the sample (b) converting the mRNA from step (a) with reverse transcriptase under conditions allowing for the production of cDNA; ? amplifying the cDNA from step (b) by polymerase chain reaction to form an amplified PCR product (d) converting the amplified PCR product from step ? with a pair of nested primers to form a nested labelled PCR streptavidin-coated microtiter plate; (f) adding a peroxidase substrate to the immobilised product from step (e) to produce a coloured reaction product; and (g) detecting the resulting coloured reaction product using an ELISA reader. | |
Technology | Virology, Diagnostic |
Inventor | Khatijah Yusoff, Kho Chiew Ling, Mohd Azmi Mohd Lila, Siti Suri Arshad |
Statement of Novelty | |
Work Start Date | |
Work End Date | |
Classification | |
Commercialised Status | |
Faculty | Biotechnology and Biomolecul Sciences |